一、人胚胎干细胞建系技术

   

NS-1，p8，x10

充分扩展的囊胚

hES细胞表面标记物鉴定

hES细胞体内分化为含三个胚层的畸胎瘤

hES核型分析

三、人胚胎干细胞定向分化技术

Neurospheres of differentiated (×200）

β-tubulin-III immuno-positive neurons (×400)

GFAP immuno-positive astrocytes (×400)

GALC immuno-positive oligodendrocyte (×200)

四、家兔体细胞克隆（核移植）技术

五、胰岛分离技术

A Protocol for Islet Isolation from Mouse Pancreas

Nature Protocols  | VOL.4 NO.11 | 2009 | 1651

Figure  | Isolated mouse islets. The photos show the islets kept in cell culture medium (a, b, c) and the islets after dithizone staining (d, e, f). (a, d) Islets isolated through the present procedure before hand-picking. (b, e) Incompletely digested islets for troubleshooting. (c, f) Purified islets after hand-picking. The photos show the islets kept in the culture medium (top row) and the islets after dithizone staining (bottom row). Scale bars represent 150 μm.

六、胰岛移植技术

Figure. Blood glucose monitoring in diabetic mice. STZ-induced diabetic mice were maintained in the institutional animal center with free access to water and chow. Blood glucose concentration was continuously assessed and presented on control animals (n=4, gray  lines) and islet-transplanted animals (n=7, black lines) respectively. Female C57BL/6J mice were used as recipients and male C57BL/6J as donors. 400 islets were subcapsularly transplanted. Tx: islet transplantation; Nc: nephrectomy.

小鼠肾被膜下移植胰岛免疫组化分析

七、基于干细胞的艾滋病基因治疗

CCR5基因靶向锌指酶mRNA转染

   Cel-1分析转染的hES细胞,结果显示大约27％的CCR5基因发生了突变(右，a)  a、b、d：锌指酶mRNA转染；c：锌指酶质粒DNA转染

八、基于干细胞的肿瘤基因治疗

 MSC^PTEN细胞分泌的PTEN-RFP融合蛋白进入邻近细胞的显微镜观察  图中4个图片取自同一个培养皿的同一个视野。第一天将CFSE（绿色荧光染料）染色的胰腺癌细胞 (Panc-1) 种植在培养皿内，第二天将MSC^PTEN细胞加种到同一个培养皿内，第三天做显微镜检测。M:已经开始表达PTEN-RFP (RFP, 红色荧光蛋白) 融合蛋白的MSC^PTEN细胞；M: 还没有开始表达PTEN-RFP的MSC^PTEN细胞；P: CFSE 染色的Panc-1癌细胞。黄色箭头指示含有PTEN-RFP融合蛋白的癌细胞。

九、微速摄影技术（Time-Lapse）

本研究所拥有国内迄今为止为数不多的微速摄影系统，由高级Leica荧光倒置显微镜、三微全自动控制的载物台、CO2培养箱、恒温控制仪等组成，可以对体外培养的细胞进行多点、任意定时高清晰自动连续拍照数千张（通常为3～5天），然后，通过电脑软件将这些照片制作成电影，动态观察细胞生长情况。